UKE Microscopy Imaging Facility (UMIF)

UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)

MINFLUX SARS-CoV2

MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible.

(1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM,

(4) schematic overlay with SARS-CoV2 model

CONFOCAL MICROSCOPY III

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus).

Author: Prof Saez

CONFOCAL MICROSCOPY I

Optical nerve

Tiled scan

CONFOCAL MICROSCOPY II

Heart tissue section

SUPER-RESOLUTION IMAGE OF DETYROSINATED TUBULIN

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 2D dSTORM image of nuclear pore complex (NPC)

NPC with Abberior FLUX640

STRUCTURED ILLUMINATION MICROSCOPY (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

COMBINED SD-TIRF IMAGING OF CELLULAR VESICLES

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

SUPER-RESOLUTION RADIAL FLUORESCENCE MICROSCOPY (SRRF)

Multi-color HeLa cells

green = F-Actin, red = alpha-Tubulin, gray = TOMM-20

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Latest news and events

Zeiss workshop at UMIF

Dear Users,

Since we are planning to acquire a new, state-of-the-art confocal microscope in 2027, Zeiss will show us their new confocal microscope setup between May 6th and May 13th.

In addition to the Airyscan 2, Multiplex, and spectral imaging, which are already available in-house, this machine will come with the new Lightfield 4D, which promises to revolutionize volumetric, high-speed imaging in scientific applications.  This new imaging capability is ideal for organoid, organs and tissue imaging, and it allows for the recording of large imaging volumes in a short amount of time.

Please don't miss your chance to test these cutting-edge tools and provide us with your evaluation!

ZEISS Technology Demonstrations 1

Read the info in the flyer, and book your imaging section using the link near the barcode. Please note that the barcode may not be working yet.

Book your section, and ask Zeiss how to best prepare your sample.


Please share this information with anyone who might be interested. 

Best wishes

Antonio Virgilio Failla

Leica workshop at UMIF


Hello UMIF friends! We would like to announce an exciting Leica workshop!

Leica will bring the best of their new microscopes to UKE between April 20 and 29.

During this time, you will have the opportunity to test:
a) Their most advanced confocal system (Stellaris), equipped with a STED unit. It's a great opportunity to push the system's resolution to its limits with your samples. 
b) It will also be possible to test their spinning disk setup, which is ideal for very fast cell imaging, also in live conditions.
c) Leica will also demonstrate the power of Aivia, Leica's AI-based image analysis software.

To prepare for this exciting demonstration, Leica will offer a very instructive webinar on April 14th from 10:00 a.m. to 12:30 p.m., during which all of the machines presented during the workshop will be introduced.

Link for the webinar: https://teams.microsoft.com/meet/23773178577575?p=kDOHHavQoNFNFfU2QM


Please share this information with anyone who might be interested. This is a unique chance to get inspired and create a positive connection with the imaging facility. If we understand your research needs, we will try to meet them.

All the information about the workshop and how to register are in the flyers. We look forward to seeing many of you at the workshop!


UMIF celebrates its 15th anniversary with a fresh new logo

Dear UMIF family, 

As part of the 15th UMIF birthday, we've upgraded our logo. We decided on a modern look with a pinch of history. Below a little reveal clip. Stay tuned for more action! Tschüss:)

MI-SIM Super-Resolution Workshop

Dear users,

I would like to let you know, that following up the very interesting webinar, CRS Biotech will bring their super-resolution live-cell imaging platform to UKE.

We would love you to challenge this system with your samples. It claims to achieve 60 nm optical resolution in live-cell imaging condition.

There are still some slot free and it would be a pity to not use them all.

Please reserve your demo slot by using the QR code present in the attached flyer and do not hesitate to contact me if you have questions about the sample preparation.

Please diffuse this information in your network and inform anyone who might be interested. You are all warmly invited <3

 

 

 

Super resolution light nanoscopy at the single molecule level workshop (09-13.02.2026)

The UKE Microscopy Imaging Facility (UMIF), Abberior Instruments, Abberior Dyes and Abbelight invite you to:


Get inspired by cutting-edge applications of super resolution light nanoscopy in biomedical research.
Learn how to design samples and the labels available for super-resolution light nanoscopy.


Get familiar with the most advanced super-resolution techniques: STED, camera-based SMLM and MINFLUX nanoscopy.


Go to the microscopes for hands-on sessions!


Book a new experience with the MIRAVA Polyscope, our special guest for the entire workshop.

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