UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)


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MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible. (1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM, (4) schematic overlay with SARS-CoV2 model

Confocal microscopy III

Mouse hippocampus

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus). Author: Prof Saez

Confocal microscopy I

Optical nerve

tiled scan

Confocal microscopy II

Heart tissue section

Super-resolution image of detyrosinated tubulin.

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 3D dSTORM image of microtubules

Alpha-tubulin in HeLa cells.

Color-coded z-distribution.

Structured illumination microscopy (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

Combined SD-TIRF imaging of cellular vesicles

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

Super-resolution radial fluorescence microscopy (SRRF)

Mutli-color HeLa cells

green = F-Actin red = alpha-Tubulin gray = TOMM-20

3i imaging workshop

This September we are testing an advanced spinning disk system, which configuration can be customized and will be specialized in long term high spatial/temporal resolution live cell imaging. The company that produced this microscope is 3i (see flyer below).
Practical microscopy sections will be held between 06.09-2021 and 10-09.2021, however, please join our webinar that will be take place the 02.09 at 1 PM in order to understand the potential of the presented microscope and eventually consider to prepare a testing sample.

In details, the access to the webinar will be granted by connection to Dr. Failla's webex room: the 02.09 at 1 pm. All of you and every one you think it might be interested are warmly invited.
Please send Dr. Failla a feedback email (This email address is being protected from spambots. You need JavaScript enabled to view it.) if you plan to join the webinar.
In case you want to reserve a testing slot between 06.09-2021 and 10-09.2021 please contact Dr. Failla at This email address is being protected from spambots. You need JavaScript enabled to view it. and try to insert your name in this calendar:

Once again you are all warmly invited to participate and your contribution is essential: Please help us to help you in making our research environment even more powerful.


3i workshop

Basic and Advanced Light Microscopy Workshop March 2021

You can register now for our two regular Light Microscopy Workshops:


03.03.2021: UMIF Basics of Light Microscopy Workshop (Registration deadline: 01.03.2021)

12.03.2021: UMIF Advanced Light Microscopy Workshop (Registration deadline: 10.03.2021)


Check out the event details pages for further information and registration.


Facility rooms closed until 22.03.2020

In the need to introduce hygienic procedures in order to ensure safe working conditions we close the facility rooms till Sunday 22.03 included.

Facility services as experiments planning, microscope purchasing support etc. etc. will go on, please do not hesitate for any of your requirements to call or email me.

Thank you for your understanding and cooperation.

Best wishes

Antonio Virgilio Failla

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