10x CFI Plan Fluor DL Phase

NA: 0.30

WD (mm): 15.2

Pixel size in image (µm): 1.100

20x CFI Plan Fluor DLL Phase

NA: 0.51

WD (mm): 2.1

Pixel size in image (µm): 0.550

40x Plan Fluor Phase

NA: 0.75

WD (mm): 0.66

Pixel size in image (µm): 0.275

40x CFI Plan Fluor Oil

NA: 1.30

WD (mm): 0.2

Pixel size in image (µm): 0.275

60x Apo TIRF (corr.) Oil

NA: 1.49

WD (mm): 0.13 (CS: 0.10-0.22 @ 23 or 37 °C)

Pixel size in image (µm): SpinningDisk: 0.183; SORA disk: 0.065

100x CFI Plan Apo Lambda

NA: 1.45

WD (mm): 0.13

Pixel size in image (µm): spinningDisk: 0.110; SORA disk: 0.039

2x Photometrics Prime 95B (back-illuminated sCMOS, 11µm pixel-size, 1200x1200 pixels)

Solid-state: 405 / 445 / 488 / 515 / 561 / 640

Yokogawa CSU W-1 SoRa in dual-camera configuration

1) Confocal disk (50µm pinholes)

2) SoRa disk for super-resolution

Camera 1:

• DAPI, 460/50
• CFP, 470/24
• GFP, 525/50

Camera 2:

• YFP, 535/30
• mCherry, 609/54
• CY5, 700/75

405/488/561/640 (used with the respective channels)
445/515/640 (used for 445, 515 and 640 channels)

561LP
514LP

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

• DAPI (blue; DAPI):
  BP 325-375 | DM 400 | BP 435-485

• GFP (green; AF488, GFP):
  BP 450-490 | DM 495 | BP 500-550

• TxRed (red; AF568, mCherry):
  BP 540-580 | DM 585 | BP 593-668

TIRF-FRAP1:

• 405/488/561/640 rpc BS (Chroma; 2mm)

TIRF-FRAP2:

• 442/514 rpc BS (Chroma; 2mm)