UKE Microscopy Imaging Facility (DFG Research Infrastructure Portal: RI_00489)


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MINFLUX microscopy made the visualization of SARS-CoV2 virus molecules possible. (1) Confocal, (2) MINFLUX, (3) MINFLUX superimposed with EM, (4) schematic overlay with SARS-CoV2 model

Confocal microscopy III

Mouse hippocampus

GFAP (for astrocytes) / Iba-1 (microglia) / Hoechst (nucleus). Author: Prof Saez

Confocal microscopy I

Optical nerve

tiled scan

Confocal microscopy II

Heart tissue section

Super-resolution image of detyrosinated tubulin.

Detyrosinated tubulin in neurons.

left = confocal, right = STED

Super-resolution 3D dSTORM image of microtubules

Alpha-tubulin in HeLa cells.

Color-coded z-distribution.

Structured illumination microscopy (SIM)

Multicolor labeling of an eukaryotic cell.

red =actin, green = focal adhesions, blue = DAPI

Combined SD-TIRF imaging of cellular vesicles

3D reconstruction of CLN3-vesicles and lysosomes in HeLa cells.

green = CLN3-GFP, red = lysosomes

Super-resolution radial fluorescence microscopy (SRRF)

Mutli-color HeLa cells

green = F-Actin red = alpha-Tubulin gray = TOMM-20

M Squared workshop

In the frame of the UMIF workshop series 2021/2022, there will be an exciting webinar about the AURORA Airy Beam Light Sheet Microscope presented by Dr Valentina Lo Schiavo from M Squared Life.

It will take place on 24th November 2021 @ 1 p.m., and you can register via the form >> HERE << to receive the webinar link.

The AURORA Light Sheet microscope enables 3D volumetric of thick specimen. If you are interested, please register and share this information.

Msquared webinar

Sysmex slide scanner workshop

In the frame of our 2021/2022 workshop series, UMIF is hosting the Sysmex Pannoramic MIDI II slide scanner microscope. This is a high content screening system that allows whole slide scanning in brightfiled and fluorescence mode.
If you are interested in this system, you are warmly invited to test it with your own samples:

Hands-on sessions: Possible between 20. October - 19. November (please contact Dr. Antonio Virgilio Failla, This email address is being protected from spambots. You need JavaScript enabled to view it.)

Please click on the flyer to download the high-resolution PDF version.


sysmex workshop

Nikon AX R confocal microscope system

This month, UMIF will host Nikon to test their next generation confocal microscope - the Nikon AX R. It features 8K x 8K resolution, 2K x 2K ultra-high speed resonant scanning, and world’s largest 25 mm field of view in combination with a new interface and advanced AI-based tools. 

We look forward to evaluating the microscope's performance with selected groups at UKE/Campus Forschung from September 20.-24.

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