Microscope

Olympus IX-83

Objectives

• 100x UPlan Apo HR TIRF Oil (NA: 1.49; WD (mm): 0.11; CS: 0.13-0.19 @ 37 °C; pixel size in image (µm): 0.96)
• 150x UPlan Apo HR TIRF Oil (NA: 1.45; WD (mm): 0.11; CS: 0.13-0.19)
• 60x UPlan Apo Oil (NA: 1.45; WD (mm): 1.1; CS: 0.13 -0.19)
• 40x UPlan Apo (NA: 0.9; WD (mm): 0.20; CS: 0.11-0.23)
• 10x UPlanFL N (NA: 0.3); WD (mm): 10

Cameras

2x Hamamatsu 1.33/C14440-20UP

• Quantum efficiency: 0.81
• Photon conversion factor: 0.24

Laser lines (nm)

Solid-state: 

• SMLM acquisition: 640
• Widefield imaging: 561 / 488
• fluorophore reactivation: 405

Filter wheels 

• ZET405-488-561-640
• ET445(58)
• ET525(50)
• ET600(50)
• ET700(75)

Acquisition software

Abbelight Neo acquisition

Analysis software

Abbelight NEO analysis

Image scanning technology

Adaptative Scanning for Tuneable Excitation Regions (ASTER)

TIRF light path 

TIRF translational stage for epi, HiLO & TIRF excitation

2D SMLM

Abbelight SAFe 180/360, simultaneous dual-color, spectral demixing

3D SMLM extension

Abbelight SAFe 360 with astigmatism lens

Hardware autofocus

IX3-ZDC

Actively damped optical table

Newport Vision IsoStation