DFG code: INST 152/1084-1 FUGG
Location: Campus Forschung II (N25), room 99.1.020.3, basement first floor
Excitation laser lines: 405, pulsed 440 nm, pulsed 485 nm, pulsed 518 nm, pulsed 561 nm, pulsed 640 nm, pulsed 685 nm
STED laser lines: 589nm, 775nm
Imaging modalities: confocal, MATRIX, RAYSHAPE, STED
Software: LIGHTBOX (including TRUESHARP deconvolution and TIMEBOW package)
Live cell imaging: Add-on environmental incubation system from OKO LAB with temperature, humidity and CO2 control
STED publications @ UMIF: [docx]
| Microscope | Olympus/Evident IX83 with z-drift compensation unit |
| Objectives |
60x UPLX-Apo Oil NA: 1.42 WD (mm): 0.15 |
| Detectors | 3xAPDs, 3 spectral detection units (400nm - 800nm), MATRIX spectral detection unit |
| Excitation laser lines [nm] |
405nm, pulsed 440 nm, pulsed 485 nm, pulsed 518 nm, pulsed 561 nm, pulsed 640 nm, pulsed 685 nm
|
| STED laser lines |
|
| Pinhole size | Motorized pinhole with 18 diameters |
| STED resolution | 80x80x90nm in 3D; 25x25nm in 2D |
| Filters for epi-fluorescence |
Filtersystem (em.-color, dye): excitation
|
| UV-VIS illumination | LED spectrum: 365-770 nm, with 16 LEDs |
| CO2 controller | Okolab |
| Temperature controller | Okolab |
| Software | LIGHTBOX software with TRUESHARP and TIMEBOW |
| easy 3D |
Spatial light modulators for 3D STED @ 595 & 775 nm and resolution <100x100x100nm in XYZ |
| RESCue STED | for bleaching reduction |
| DyMIN STED | Light dose management for super-resolution live imaging |
| MINFIELD STED | Light dose management for higher contrast and resolution |
| Quad-Scanner | 512x512 @ 4.2 fps; max. 16000x16000 px |
| FLIM/FCS/FCCS | TIMEBOW software + Picoquant Miltiharp 150 4P |
| Actively damped optical table | MIRAVA table frame |
| Perfusion unit | Automated Fluigent Aria |
