Location: Campus Forschung II (N25), room 99.1.020.4, basement first floor
Laser lines: 405nm/445nm/488nm/515nm/561nm/640nm
Software: VisiView
Live cell Imaging: Add-on environmental chamber with temperature, humidity and CO2 control
| Microscope | Nikon Eclipse TiE |
| Objectives |
10x CFI Plan Fluor DL Phase NA: 0.30 WD (mm): 15.2 Pixel size in image (µm): 1.100 20x CFI Plan Fluor DLL Phase NA: 0.51 WD (mm): 2.1 Pixel size in image (µm): 0.550 40x Plan Fluor Phase NA: 0.75 WD (mm): 0.66 Pixel size in image (µm): 0.275 40x CFI Plan Fluor Oil NA: 1.30 WD (mm): 0.2 Pixel size in image (µm): 0.275 60x Apo TIRF (corr.) Oil NA: 1.49 WD (mm): 0.13 (CS: 0.10-0.22 @ 23 or 37 °C) Pixel size in image (µm): SpinningDisk: 0.183; SORA disk: 0.065 100x CFI Plan Apo Lambda NA: 1.45 WD (mm): 0.13 Pixel size in image (µm): spinningDisk: 0.110; SORA disk: 0.039 |
| Cameras |
2x Photometrics Prime 95B (back-illuminated sCMOS, 11µm pixel-size, 1200x1200 pixels) |
| Laser lines (nm) |
Solid-state: 405 / 445 / 488 / 515 / 561 / 640 |
| Spinning Disk unit |
Yokogawa CSU W-1 SoRa in dual-camera configuration 1) Confocal disk (50µm pinholes) 2) SoRa disk for super-resolution |
| Emission filters spinning disk (for laser-related fluorescence) |
Camera 1:
Camera 2:
|
| Dichroic in spinning disk unit |
405/488/561/640 (used with the respective channels) |
| Dichroic for dual camera mode |
561LP |
| Fluorescence filters in microscope stand |
Filtersystem (em.-color, dye): excitation | beamsplitter | emission
|
| Dichroic mirrors in microscope stand for TIRF and FRAP |
TIRF-FRAP1:
TIRF-FRAP2:
|
| UV-VIS illumination | SOLA-SM Light Engines, white light LED, 380-680nm |
| Transmitted light lamp | precisExcite, High-Power 525nm LED |
| Software | VisiView v4 |
| Incubation unit | okolab bold line |
| FRAP-TIRF unit | Roper iLAS2 for FRAP&TIRF with all laser lines |
| Piezo focus drive | Ludl NanoPrecision PiezoZ, 350µm travel range |
| Motorized XY stage | Ludl BioPrecision3 LM |
| Auto-focus | Nikon PerfectFocus system |
| Actively damped optical table | Newport |
