Systems

Aurox SD

Aurox SD

Location: Campus Forschung (N27), room 02.053, second floor

Fluorescence: DAPI / GFP / RFP / Far-Red

Software: Visionary

Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

MicroscopeZeiss Axiovert 200M
Objectives

10x EC Plan-Neofluar

NA: 0.3

WD (mm): 5.2

Pixel size in image (µm): 0.65


20x EC Plan-Neofluar

NA: 0.5

WD (mm): 2.0

Pixel size in image (µm): 0.325


10x EC Plan-Neofluar Ph1

NA: 0.3

WD (mm): 5.2

Pixel size in image (µm): 0.65


20x EC Plan-Neofluar Ph2

NA: 0.5

WD (mm): 2.0

Pixel size in image (µm): 0.325


63x Plan-Apochromat Ph3 Oil

NA: 1.4

WD (mm): 0.19

Pixel size in image (µm): 0.103

Camera1x Photometrics BSI Express
Spinning Disk unitAurox Clarity
Emission filters spinning disk (for image acquisition)

blue (DAPI): Ex 392/23, Em 447/60
green (GFP), Ex 466/40, Em 525/45
red (DsRed), Ex 554/23, Em 609/54
far-red, (CY5), Ex 635/18, Em 680/42

Fluorescence filters in microscope stand

Filtersystem (em.-color, dye): excitation | beamsplitter | emission

  • 49 (blue; DAPI):
    G 365 | FT 395 | BP 445/50

  • 09 (green; AF488, GFP):
    BP 450-490 | FT 510 | LP 515

  • 43 (red; AF568, mCherry):
    BP 545/25 | FT 570 | BP 605/70

UV-VIS illumination

For visual inspection: EXFO X-cite 120

For imaging: CoolLED pE-4000, 16 LEDs for excitation in the range 365-770 nm

Halogen lamp100W, standard housing
SoftwareVisionary
Incubation unitSolent Scientific
Piezo focus driveLudl Electronic Products PiezoZ, 200µm travel range
Motorized XY stage

Ludl Electronic Products BioPrecision

Actively damped optical tableNewport

Tags

FRET, Live imaging, Spinning Disk, SRRF, Super resolution, Wide field
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